House Fly – BASD

May 5, 2026

Sample information
Picture
Entry by: Caleb K
Location
Collection date 08/26/2025
Captive / Cultivated?
Group Beaver Area School District
Observations

I collected my fly from the grill of my teacher’s car, the temperature was approximately 58 degrees F, and was scooped into a small tube with a Q-tip.
Putative identification

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

I made sure to crush the bug as much as possible and carefully complete all the PCR steps.

Results
Wolbachia presence
Confidence level
Explanation of confidence level

The insect is most likely a negative, there was a band in a negative lane, which nulls any data found.

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Wolbachia 16S sequence
Arthropod COI sequence
Summary
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