House Fly – BASD

May 5, 2026

Sample information
Picture
Entry by: Caleb K.
Location
Collection date 08/26/2025
Captive / Cultivated?
Group Beaver Area School District
Observations

The fly was found malformed on my teacher’s car’s grill. The temperature was roughly 58 degrees F, and it was scooped into a small tube.
Putative identification Arthropoda Insecta

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system BioRad
Buffer TAE
DNA stain UView
Gel images
Protocol notes

The bug was thoroughly crushed up and all PCR steps were done as usual.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

There was a faint band in a negative lane, so my data is a little unreliable.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be negative for Wolbachia.
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