Sample information
Picture	Entry by: Amelia S.
Location
Collection date	09/03/2024
Captive / Cultivated?	Wild-caught
Group	Benedictine University
Observations	Caught in the fall of 2024
Putative identification	Arthropoda Insecta Orthoptera
Methods
Extraction kit	Blood and Tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	My controls worked, other samples on same gel came back positive
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Orthoptera was found to be negative for Wolbachia.