Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/02/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | collected by Fall 2025 students |
| Putative identification | Arthropoda Insecta Dermaptera Forficulidae Forficula Forficula auricularia |
Methods |
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| Extraction kit | Qiagen DNeasy Blood and Tissue Kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes |
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Results |
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| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Gel Machine did not run correctly and gel bands did not separate. Therefore no identifiable results could be concluded. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
>29_E_AG1_CoAF (editing).scf (449 bp) NGNCCCCANATANNGNNNNNACNCCCNNANATGNNANNNNNNNNNNTTTTACCTCCTGNNNNNNTGNATNNNNCNNNATT NNATNNTNNNNNNNNNNNCTGGNNANNGNNAGANNNNGGCCNCGTCNCCCTCCTANANANTNTACCCCCCNANNNNGNNN NNNNNNNNNNNNNTNTNTNTTTNTNANNCANGAGANTNTTCNTNNATTGTATNTGNNAGGNTTTNNNTANATTNNNNNNN NAATANNNNNANTCANNACNNNNNNNNANNNNNNNNNNNNGNNNCNTNANNTGNANNNNNNNNNGANNNTTTGTATGATC ATTANNNNNNNNNGCTCANNTATTGNTGTNCNNNTNNNNNANTAATGNCANAATNNNNNNNNNTNNNTTNTGNCNNANCN NGNNNNNCAANCNNNANNNTNNNNNNNNTNGNNNTNNGNCGCCCCATTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR