Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/03/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Benedictine University |
| Observations | |
| Putative identification | Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus |
Methods |
|
| Extraction kit | Qiagen DNeasy #69504 |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | LB |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The results from the Gel and the BLAST results prove that there was presence of Wolbachia in the arthropod |
| Wolbachia 16S sequence | Download FASTA
|
| Arthropod COI sequence | Download FASTA
|
| Summary | The Oncopeltus fasciatus was found to be postive for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR