Oncopeltus fasciatus (Large Milkweed bug) – Shahid Hameed

May 11, 2026

Sample information
Picture
Entry by: Shahid H.
Location
Collection date 09/18/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

collected by Fall 2025 students
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Oncopeltus Oncopeltus fasciatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I concluded that my confidence level was high since I had a thick Wolbachia gel band and a 100% ID match in BLAST.
Wolbachia 16S sequence Download FASTA   
Arthropod COI sequence Download FASTA   
Summary The Oncopeltus fasciatus was found to be postive for Wolbachia.
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