Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/25/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | 6 legs, 2 feelers, 2 wings |
| Putative identification | Arthropoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys |
Methods |
|
| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | agarose gel electrophoresis |
| Buffer | 1X TAE |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The controls worked perfectly. I would say the only reason to question the results is because another round of PCR may need to be done to know for sure that there is no Wolbachia present. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Halyomorpha halys was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR