Pill Bug GROUP2

May 22, 2026

Sample information
Picture
Entry by: MJ
Location
Collection date 05/01/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations
  • Found in CAMS bushes
  • Dark brown with small white patterns
  • Moves slowly
  • Has many legs (~ 13)

 
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Edwards Buffer was used in the DNA extraction

Duplex PCR performed with the cocktail and F/R primers

Gel electrophoresis with 1x TAE buffer and ran at 120V, 65 mA

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Gel showed only two distinct bands in lanes 2 and 6. The DNA Ladder, (+), and (-) Arthropod controls were not visible.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
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