Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/15/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | California Academy of Mathematics and Science |
| Observations | Found in outside bush area hidden near branches |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | QIAGEN DNeasy kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system Bio-Rad |
| Buffer | 1X TAE |
| DNA stain | 100X Fast Blast |
| Gel images |
|
| Protocol notes | 1% agarose gel ran at 165 volts, 75 mAmps, for 65 min
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Well #2 containing the spider sample visualized no results meaning an error in PCR or DNA extraction, likely being the latter. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |


Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR