Argenna Spider GRP 9

May 21, 2026

Sample information
Picture
Entry by: Erinne P
Location
Collection date 04/30/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Found outside in grassy area
Putative identification Arthropoda

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system Bio-Rad
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Nothing appeared in the positive and negative wolbachia wells; therefore wolbachia cannot be confirmed. However, the DNA only had one band that matched the DNA+ control, leading us to believe that the DNA indicated arthropod status and not Wolbachia presence.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGATCTTGATCTNGCTATATTAGGTACTGCTATAAGTGTTTTAATTCGGATGGAATTANNNNNNN NNGNNNATTGTTAGGAGATGATCATTTATATAATGTAATTGTTACTGCTCATGCTTTTGTTATGA TTTTTTTTATAGTAATGCCTATTATAATTGGGGGGTTTGGTAATTGATTAGTTCCTTTAATGTTA GGAGCTCCGGATATAGCTTTTCCTCGTATAAATAATTTAAGATTTTGATTATTACCGCCATCATT AATCTTATTATTGATTTCATCTATAGTTGAAATAGGAGTAGGAGCTGGTTGGACTGTATATCCTC CTTTAGCTTCTTTGGAAGGTCATGCAGGATCGGCAATGGATTTTGCTATTTTTTCTTTACATTTA GCTGGGGCATCTTCTATTATAGGGGCGATTAATTTTATTTCTACTATTATTAATATACGTTCCGT TGGAATGAGAATGGAAAGGGTTTCTTTATTTGTGTGGTCTGTGTTGATTACTGCTATTTTATTAT TATTATCTTTACCAGTATTAGCTGGTGCTATTACTATATTGTTGACTGATCGTAATTTTAATACA TCTTTTTTTGATCCTGCTGGGGGAGGAGATCCTATTTTATTTCAGCATTTATTTTGATTTTTTGG TCACCCTGGAAGTTTAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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