Armadillidium Vulgare GRP11 – Arthropod Male Sample #1 (AM1) | Carson, California, 90746

May 21, 2026

Sample information
Picture
Entry by: NC, OS, AL, YL, CE, JP
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group California Academy of Mathematics and Science
Observations

Caught outside classroom around 9:00 am in bushes. This pill bug was average-size compared to other observed pill bugs.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Edwards Buffer
DNA extraction location Other
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain UView
Gel images
Protocol notes

Used UV Stain. UV light right after. 10 microliters of sample were added per well for this 1% agarose gel.

Lane order of left gel (left to right):

Well 1  Control (-)
1 Band
Well 2 Ladder 
5 Bands
Well 3 Arthropod Sample 1 (Female)
1 Band
Well 4 Arthropod Sample 2 (Male) (THIS SAMPLE)
Smearing
Well 5 Arthropod Sample 3 (Male)
Smearing & 1 Band
Well 6 DNA Control (+)
1 Band
Well 7 Control (+)
1 Band
Well 8 N/A
N/A

Lane order of right gel (left to right):

Well 1  Control (-)
No Bands
Well 2 Ladder
5 Bands
Well 3 Arthropod Sample 1 (Female)
Smeared Band
Well 4 Arthropod Sample 2 (Male)
Smeared Band
Well 5 Arthropod Sample 3 (Male) (THIS SAMPLE)
Smeared Band
Well 6  DNA Control (+)
1 Band
Well 7 Control (+)
1 Band
Well 8 N/A
N/A

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

On the gel to the left, we had analyzed our samples for arthropod positivity. In this gel, the AM1 sample was ran in well 4 and did not portray a correlating band to the arthropod controls in wells 1 and 6. With no clear band visible for the AM1 sample in well 4, we are not confident that the AM1 sample was an arthropod.

Furthermore, we are unsure of the Wolbachia positivity for the AM1 sample due to our results seen in the gel to the right. In this gel, the AM1 sample (Well 4) did not show any correlating bands with the Wolbachia positive control (Well 6). Additionally, Well 4 portrayed smearing throughout the gel, which is a large factor in lowering our confidence level. Since only a smeared band could be identified, we are not confident about the Wolbachia positivity of the AM1 sample as well as its negativity.

Because there was a lack of clear results / bands in both gels for AM1, we were unable to sequence the DNA.
Wolbachia 16S sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
N/A
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
Report Inappropriate Post