Lasius brunneus

May 21, 2026

Sample information
Picture
Entry by: Magdalena V & Yara L.
Location
Collection date 03/31/2026
Captive / Cultivated? Wild-caught
Group Gymnasium Immensee
Observations

Captured on a gravel path next to a forest.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius brunneus

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TAE
DNA stain SYBR Green
Gel images
Protocol notes

The following lane order for our gel:

1st from left: DNA from other insect
2nd left: DNA lasius brunneus
3rd from left: positive arthropod control
4th  from left: negativ arthropod control
5th from left: gDNA
6th  from left: water
7th from left: DNA marker

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

All of the controls worked except the negative arthropod control.

For the first and second ones (see reference above “protocol notes”) were used 400 microliters insted of 50 microliter TE buffer for the DNA-Elution. It had no influence on the results, because they were still clearly visible.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lasius brunneus was found to be negative for Wolbachia.
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