Sample information

Found under rotting wood in damp soil in same colony as Isopod #1. It was caught at around 4:40 pm, and the temperature outside was 5°C.

Methods

DNA Extraction – This isopod was larger than the first. The lower half of the abdomen was removed and the exoskeleton was scored with a scalpel as well. It was crushed very well with no large pieces or major discolouration.

Gel Electrophoresis – For CO1 arthropod

The first two wells got damaged so we had to move over:

3: NEB NO551L Ladder

4: Isopod #1

5: Isopod #2

6: Isopod-R #1

7: Isopod-R #2

8:

9:

10: Positive Control

11: Negative Control

12: DNA Postive Control

13: Water / DNA Negative Control

Wolbachia 16S

1. NEB NO551L Ladder

2. Isopod #1

3. Isopod #2

4.

5.

6. Isopod-R #1

7. Isopod-R#2

8.

9.

10. Positive Control

11. Negative Control

12. DNA Positive Control

13. Water / DNA Negative Control

Analysis: Our controls did not work/were contaminated, during Gel Electrophoresis, the Arthropod CO1 Band was present and the Wolbachia 16S was not present, however these results cannot be trusted to do low confidence level. There was likely an error in the DNA Extraction and PCR steps.

Results

Our water (DNA – control) was contaminated with DNA, and our Wolbachia positive control did not indicate presence of Wolbachia. Therefore our confidence level is low and our results cannot be trusted.