Sample information

Found under rotting wood on rocks with a different colony than Isopods 1 and 2 in the soil. It was 8:00pm and it was 14°C.

Methods

DNA Extraction – This isopod was medium sized, a bit larger than Isopod #1, its abdomen was cut in the middle and its exoskeleton was scored, it was harder than the first two. It was crushed well but small pieces of exoskeleton remained uncrushed, there was a slight pink discolouration in the liquid as well.

Gel Electrophoresis – For CO1 arthropod

The first two wells got damaged so we had to move over:

3: NEB NO551L Ladder

4: Isopod #1

5: Isopod #2

6: Isopod-R #1

7: Isopod-R #2

8:

9:

10: Positive Control

11: Negative Control

12: DNA Postive Control

13: Water / DNA Negative Control

Wolbachia 16S

1. NEB NO551L Ladder

2. Isopod #1

3. Isopod #2

4.

5.

6. Isopod-R #1

7. Isopod-R#2

8.

9.

10. Positive Control

11. Negative Control

12. DNA Positive Control

13. Water / DNA Negative Control

Analysis: Our controls did not work, during Gel Electrophoresis there was no Arthropod CO1 Band and no Wolbachia 16S band. However, due to low confidence levels, these results cannot be trusted.

Results

Our water (DNA – control) was contaminated with DNA, and our Wolbachia positive control did not indicate presence of Wolbachia. Therefore our confidence level is low and our results cannot be trusted.