Sample information
Picture	Entry by: Gracie C.
Location
Collection date	04/21/2026
Captive / Cultivated?	Wild-caught
Group	Winterset High School
Observations	A medium size black ant, 6 legs, 3 body parts
Putative identification	Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus pennsylvanicus
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	1X TBE
DNA stain	Cyber Green
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	I believe I did everything correctly.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Camponotus pennsylvanicus was found to be postive for Wolbachia.