Sample information
Picture	Entry by: Max B
Location
Collection date	04/21/2026
Captive / Cultivated?	Wild-caught
Group	Winterset High School
Observations	Small Grasshopper found in grassy area.
Putative identification	Arthropoda Insecta Orthoptera Acrididae Chortophaga Chortophaga viridifasciata
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	1X TBE
DNA stain	Cyber Green
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	The band in the lane was very bright.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Chortophaga viridifasciata was found to be postive for Wolbachia.