Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/20/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Collected from a underneath a rock next to a small stream. It was found in the afternoon at around 1pm, there were several other spiders emerging into the sun with it. |
| Putative identification | Arthropoda Arachnida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Unfortunately, the gel turned out blurry and the expected positive controls did not show up as expected. Thus, we cannot accurately conclude anything from our gel results without rerunning the samples. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR