Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/17/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | DNA Subway yielded a zero mismatch result for Salticidae (Family of which jumping spiders are apart of ). It’s second result was for Paraphidippus aurantius (emerald jumping spiders) with 7 mismatches. Although there are 7 mismatches, the physical IDs between the collected sample and other emerald jumping spiders seems to match. |
| Putative identification | Arthropoda Arachnida Araneae Salticidae Paraphidippus Paraphidippus aurantius |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Top row tests for presence of CO1 gene present in arthropod DNA. Bottom row tests for presence of the wsp Wolbachia gene. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls demonstrated proper results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Paraphidippus aurantius was found to be negative for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR