Chloropid Gout Fly

Sample information

Picture
Entry by: Gayatri M
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations
Putative identification Arthropoda Insecta Diptera Chloropidae Chlorops Chlorops pumilionis

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Other
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download AB1
Summary The Chlorops pumilionis was found to be postive for Wolbachia.
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