Winter Ant

May 29, 2026

Sample information
Picture
Entry by: Daisy D
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Found on some rocks on muddy stream bank near TJHSST
Putative identification Arthropoda Insecta Hymenoptera Formicidae Lasiini

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Protocol details:

  • Extraction Kit is “DNeasy (Qiagen) blood and tissue kit”
  • PCR was “Single reaction”
  • Gel electrophoresis system is MiniPCR
  • Buffer is TBE
  • DNA stain is SYBR safe

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

We did not see a band in our positive Drosophila control, indicating a potential issue in our samples.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
TCTGAGCTGGAATAATTGGATCTTCAATAAGAATACTTATTCGTTTAGAATTAGGTACTTCAAATTCATTAATTAATAATGATCAAGTTTTTAATTCTATAGTTACTAGACATGCTTTTATTATAATTTTTTTTATAGTCATACCTTTTATAATTGGTGGATTTGGAAATTTTTTAGTTCCTTTAATACTAGGATCTCCAGATATAGCCTATCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCAATCAGTTTACTTTTATTAAGAAATTTCATTAATGACGGAGTGGGAACAGGATGAACTGTTTACCCTCCATTAGCTTCTAATATTTTTCATAATGGTCCTTCAGTTGACCTGACAATTTTTTCTCTACATATTGCAGGAATATCTTCTATTCTAGGAGCTATCAATTTTATTTCAACAATTCTAAATATACGACATAAAAATTTTTCTTCTGATAAAATCCCTTTACTTGTTTGATCTATTTTAATTACAGCAATTTTACTTCTTCTATCTTTACCAGTTCTAGCTGGAGCTATTACTATACTTTTAACTGACCGGAACCTTAATACTTCCTTTTTTGATCCCTCAGGAGGAGGAGACCCTGTTCTTTATCAACATTTATTCTGATTTTTNG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lasiini was found to be negative for Wolbachia.
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