Yellow Jacket

May 29, 2026

Sample information
Picture
Entry by: Yana B
Location
Collection date
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Alexandria, VA 22312
weatehr was in the 50s-60s Fahrenheit at the time of capture
Putative identification Arthropoda Insecta Hymenoptera Vespidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

DNA Extraction: The abdomen was cut vertically down and the fluid inside was extracted for PCR
Analysis: This sample had a very bright band but the positive control had no band while the negative control had a decently dark band, indicating a failure in the controls and maybe our procedure

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

Both our positive and negative controls failed, so our results are not as reliable as we would like for our experimental models
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Vespidae was found to be negative for Wolbachia.
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