Miner Bee

June 1, 2026

Sample information
Picture
Entry by: Kamel E
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

The bee was captured in a wooded area by a stream. There was a slight overcast with light rain on the day of capture.
Putative identification

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes
DNA extraction: I made sure to closely follow the protocol and crush up the arthropod really well, only extracting the abdomen.

Analysis: The positive control was not visible so most likely did not work.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Our positive control failed to produce a positive result,  however all other bands showed up so I can still confidently say that there is a high confidence level that my arthropod is infected with Wolbachia.
Wolbachia 16S sequence Download FASTA    Download AB1
NNGNNNNNNNGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTN
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TNNNTCTTTGCTATNTGAGCAGGNNTAATTGGAGCATCTTTAAGATTTATTATTCGTATAGAATTAAGAAATCCAGGAAATTGAATCAATAATGATCAAATCTATAATTCAATTGTAACCTCTCATGCTTTTATTATAATCTTTTTCATAGTTATACCATTCATAATCGGAGGTTTCGGAAACTGACTTACACCGTTAATATTAGGAGCGCCCGACATGGCATTTCCACGAATAAATAACATAAGATTTTGATTATTACCTCCTTCGATTTTAATTATCTTAATAAGAATAATTTTAAATTCAGGATCTGGAACAGGATGAACTGTATATCCACCACTATCATCTTATTCATTTCACCCATCATCATCAGTTGATTTAACAATTTTTTCACTACATATTGCTGGTGTATCATCAATTATAGGAGCAATTAATTTTATTGTAACTATTCTTAACATAAAAAATATCTCATTAAACTATGATCAAATACCACTATTCCCATGATCAGTATTTATTACAACAATTCTATTATTAATTTCTCTACCAGTTCTAGCTGGTGCAATTACAATATTACTATCAGATCGAAATCTAAATTCATCCTTCTTCGACCCTATAGGAGGAGGAGATCCAATTTTATACCAACATTTATTTTGATTTTNNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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