Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/09/2019 |
| Captive / Cultivated? | Wild-caught |
| Group | |
| Observations | Sample 88 |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Muscidae Phaonia Phaonia angelicae |
Methods |
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| Extraction kit | |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | |
| Explanation of confidence level | Possible Wolbachia co-infection |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAAGTGCCCAAGCCTAACCTATTCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCACTCGAA
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Phaonia angelicae was found to be postive for Wolbachia. |
Water Boatman