Palaearctic fly

May 29, 2020

Sample information
Picture
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Bordenstein Lab
Observations

Sample 75
Putative identification Arthropoda Hexapoda Insecta Diptera Muscidae Phaonia Phaonia angelicae

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level
Explanation of confidence level

Possible Wolbachia co-infection
Wolbachia 16S sequence Download FASTA    Download AB1
CAGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTTTTTTGACCCTGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Phaonia angelicae was found to be postive for Wolbachia.
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