Housefly

June 2, 2026

Sample information
Picture
Entry by: Supreetha C
Location
Collection date 04/20/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Found on the bark of a tree near a stream. It was a warm day and the fly was collected in the morning around 9:30 AM.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

DNA Extraction: Used abdomen for DNA Extraction and crushed using sand and pestle.

Analysis: Positive and negative controls worked successfully. Sample had a CO1 band but not a wsp band, signifying the housefly did not have the Wolbachia gene.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Both positive and negative controls for the CO1 gene worked as intended, however the band for the positive control for the Wolbachia gene is fainter than the expected band. This explains my medium confidence level in my results.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Diptera was found to be negative for Wolbachia.
Report Inappropriate Post