Camponotus chromaiodes (Ferruginous Carpenter Ant) – Wolbachia +

June 3, 2026

Sample information
Picture
Entry by: Anna P
Location
Collection date 05/26/2026
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

On the campus of the Pingry School Basking Ridge Campus. Was moving across a wooden bridge above a stream in a wooded area.
Putative identification Arthropoda Insecta Hymenoptera Formicidae Camponotus Camponotus chromaiodes

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Did DNA extraction with a Qiagen kit, performed PCR, used nanodrop to determine DNA concentration, used gel imaging to determine whether the DNA had arthropod and Wolbachia DNA, and purified the DNA for sequencing.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The positive and negative controls both worked as expected; there was a strong band for the positive control and no band for the negative control. Additionally, the Wolbachia amplified DNA of the ant was 100% identical to existing Wolbachia DNA.
Wolbachia 16S sequence Download FASTA    Download AB1
GTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTTTGCTATTTGATCGGGAATAGTTGGCTCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Camponotus chromaiodes was found to be postive for Wolbachia.
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