Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/22/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | This Spotted Lanternfly nymph on a tree near a small stream in Alexandria, Virginia. It was preserved through freezing and then processed around a week after collection. |
| Putative identification | Arthropoda Insecta Hemiptera Fulgoridae Lycorma Lycorma delicatula |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | This was a two-day process, one day spent doing DNA extraction and the other day spent running the PCR process. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The controls did not work, such as the positive Wolbachia control. There should have been a band, but there was no band for the sample we know for sure has Wolbachia, so there is no way to determine definitively if this arthropod specimen contained Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR