Sample information

I found this ladybug in the grass field behind Winston Knoll Collegiate. I collected this ladybug at 11:14am, it was sunny out with a temperature of 25 degrees celsius. It was caught in the spring season on May 13th, 2026.

Methods

DNA extraction: I made sure the ladybug was thoroughly crushed. I dissected the abdomen out carefully, and did not include the exoskeleton.

Gel electrophoresis lanes for CO1 arthropod PCR:

1- NEB NO551L ladder

2- Ladybug #1

3- Big Black Beetle #2

4-Ladybug #3

5- Small Black Beetle #4

6- +arthropod control

7- -Arthropod control

8- +DNA control

9- Water

Analysis: My controls worked but my sample did not have a band, meaning it does not have Wolbachia or there was an error in my DNA extraction

Results

All of my control groups worked as expected. I followed protocol and had no problem with the gel. I am not surprised by any on the bands on the gel. Therefore I am confident this ladybug is not infected with Wolbachia.