Red Ant 2

June 4, 2026

Sample information
Picture
Entry by: Shelby O
Location
Collection date 05/14/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

May 14, 11:25 am

Windy, rainy, cloudy, ~18 C
Putative identification Arthropoda Insecta Hymenoptera Formicidae Formica

Methods
Extraction kit pcr
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

We selected medium confidence because in our results from gel electrophoresis, there seems to be a bar that would signal that our arthropod has Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formica was found to be postive for Wolbachia.
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