Pepsinae (Spider Wasp, Wolbachia +)

June 5, 2026

Sample information
Picture
Entry by: Bora A
Location
Collection date 05/27/2026
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Fully black, 6 legged, winged, has antennae. Found in bug trap in the outskirts of woods behind the Pingry School, Basking Ridge, NJ. Bug trap contained dish soap, sugar, water, yeast.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

First, DNA extraction was conducted with a DNEasy Qiagen Kit. Then, PCR was done for COI and for Wolbachia with COI and Wolbachia primers. Then, 1% gel with a 1kb ladder was run at 100V, and the DNA from PCR was purified with a QIAquick kit and then sequenced.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My confidence level is high, since Wolbachia gel (bottom gel)’s lane 3 was present in the bug. Although the Wolbachia positive control gel in lane 5 of the Wolbachia gel (bottom gel) was absent, Wolbachia DNA was successfully sequenced and matched with the database, showing that the bug had Wolbachia.
Wolbachia 16S sequence Download FASTA    Download AB1
TGTCGTNNGCTCGTGTCGTGAGANGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTAAGANTAATAATTCGTATTGAATTAGGAATACCTGGTGAAGTTTTCAGAGGAGGTCAGTTTTATAATTCTATTGTAACTGCACACGCTTTTGTAATAATTTTTTTTTTTGTAATACCTTTTATAATCGGAGGTTTCGGTAATTGGTTAATTCCCTTAATATCGGGGATTCCTGACATAGCATTTCCTCGGATAAATAATATAAGATTTTGATTATTACCTCCTTCTTTATTTTTTTTAGCTTTAGGTATATTTATTGGAGAGGGGTGTGGTACGGGCTGAACTGTATATCCTCCTTTATCAACATCTATATATCAGCCAGGACCAAGAGTAAATTTATCAATTTTTTCTCTTCATTTAGCAGGGGTTTCGTCAATTATAGGTGCTGTAAATTTTATTGTAACAATTTATATATTTTGTCGTTTTTCTTTCGATAAGGTTACTTTATTTTCATGATCTGTAATACTTACAGCAATTCTTCTATTATTATCTTTACCTGTTTTAGCAGGTGCAATTACTATACTACTTTCANATCGAAATTTTAATACTACTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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