Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | Found under at the bottom of some leaves under some leaves. |
| Putative identification | Arthropoda Malacostraca Isopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | The whole of the arthropod was crushed up to extract DNA. None of our wolbachia samples showed visible bands, which may imply that no DNA was amplified or something else went wrong during our protocol. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | Our CO1 lanes all showed visible bands, meaning that DNA was amplified yet the negative control lane also shows a clear band, meaning that cross contamination occured. Also no Wolbachia lanes showed visible bands so it is unclear whether the pillbug contains Wolbachia or not. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
(no title)
Brown Pillbug
Neighboring Miner Bee
Eastern yellow jacket