Black Beetle #2

June 10, 2026

Sample information
Picture
Entry by: Rachel B.
Location
Collection date 05/13/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

Tiny black beetle

quite fast

extremely angry!
Putative identification Arthropoda Insecta Coleoptera Chrysomelidae Phyllotreta Phyllotreta cruciferae

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer 1X TBE
DNA stain SeaGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The controls did work, and compared to our positive control, this beetle does have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Phyllotreta cruciferae was found to be postive for Wolbachia.
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