Roly-Poly(Pill-bug)

June 11, 2026

Sample information
Picture
Entry by: Nihar P
Location
Collection date 04/22/2026
Captive / Cultivated? Wild-caught
Group Thomas Jefferson High School for Science and Technology
Observations

Found in shade-covered region. Roly-poly is unwinged.
Putative identification Arthropoda Malacostraca Isopoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The gel for the Roly-Poly showed no bands whatsoever, indicating that the particular lane may not have been properly loaded. Furthermore, it is more likely that there is not Wolbachia present in the organism than Wolbachia being present, so my confidence in my results is medium, although leaning towards low. The CO1 also worked, meaning I can somewhat trust the overall results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Isopoda was found to be negative for Wolbachia.
Report Inappropriate Post