Spider

September 10, 2026

Sample information
Picture
Entry by: Ayden H
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Small bodied, brown color
Putative identification Arthropoda Arachnida Araneae Thomisidae Xysticus Xysticus funestus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

From left to right:
1. DNA Ladder: 3 bright bands; dimmer bands behind
2. 1D1 Arthropod: 1 bright band; 1 dim band
3. 1D2 Arthropod: 1 bright band
4. Arthropod Control (+): 2 bright bands
5. Arthropod Control (-): No band
6. DNA Control (+): 2 bright bands
7. DNA Control (-): No band
8. Control (Nothing): No band

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls did work and I believe the experiment was preformed up to par, the subjects just happened to lack the Wolbachia virus, leading my confidence being high.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CTGCTATANTTGGTACTGCAATAAGAGTTTTGATTCAATAGAATTAGGAAATGTAGGAAGTTTATTAGGAAATGATCATT TATATAATGTGGTTGTAACAGCTCATGCTTTTGTAATGATTTTTTTTATANTAATACCTATTTTGATTGGAGGATTTGGA AATTGATTAGTACCGTTAATATTGNGAGCTCCTGATATATCTTTTCCTCGAATAAATAATTTATCTTTTTGACTACTTCC TCCTTCTTTATTTTTATTATTTATATCTTCTTTATTAAAAATGGGAGTAGGGGCTGGAGGAACTGTTTATCCCCCTTTGC CTTCAAGTGTANGTCATATGGGGGGTTCTATAAATTTTGCTATTTTTTCTCTTCATTTAGCTGGGGCTTCTTCTATTATA GGGGCTGTAAATTTTATTTCTACNATTATTAATATACGGGTGACAGGAATAAAAATATAAAAATTACCTTTATTTGTTAG GNCTGTTTTAATTACNNNTATTTTATTGCTTTTGNCTTTACNTGCATNANCAAGATCTATTACTATATTATTATCANATC GNANTTTTAATACTTCTTTTTNNGANCCTGCAAGTGGGGGGGANCCAATTNTATTTCNACATTTATTNNGATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Xysticus funestus was found to be negative for Wolbachia.
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