Red legged grasshopper (Orthoptera)

September 10, 2026

Sample information
Picture
Entry by: Huzifa Osman
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1: DNA Strand

Lane 2: HOBG1

Lane 3: HOBG2

Lane 4: EBCP2

Lane 5: HOBG3

Lane 6: HOBG4

Lane 7: DNA Control

Lane 8: Water Control

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The PCR/Gel supports it
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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