Boisea rubrolineata (western boxelder bug)

September 10, 2026

Sample information
Picture
Entry by: Ohoud M
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

tear drop shaped hard tick, brownish-black legs and dorsal sheild with a brownish or most red lower body.
Putative identification Arthropoda Insecta Hemiptera Rhopalidae Boisea Boisea rubrolineata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1 was the DNA standard/ladder , Lane 2 was OMAM01 (Boisea rubrolineata) which was an arthropod with no Wolbachia , Lane 3 was OMAM02 (Leiobunum sp.) which was also an arthropod with no Wolbachia, Lane 4 is the positive control , Lane 5 is the negative control, lane 6 is the DNA control, and Lane 7 was water which is a negative control to detect contamination.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The E-value for the nucleotide sequence we’re 0.0 meaning that the results are very significant and reliable and therefore, the confidence level is high.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TTNGNATATGATCTGGTATAGTAGGTTCATCTTTAAGATGAATTATTCGTGTAGAATTAGGGCAACCTGG TAGATTTATTGGGGATGATCAAACATATAATGTAATTGTAACAGCACATGCTTTTATTATAATTTTCTTT ATAGTTATGCCAATTATGATTGGTGGATTTGGGAATTGATTAGTGCCTTTAATAATTGGGGCCCCAGATA TAGCATTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCCCTTACTTTATTACTTGCAAG TAGTATAGTTGAAAGAGGGGCGGGAACTGGATGAACAGTTTATCCCCCTCTATCAAGAAATTTATCCCAT AGAGGTGCCTCTGTAGATTTAGCAATTTTTTCATTACATTTAGCAGGTGTATCATCAATTTTAGGGGCTA TTAATTTTATTTCAACTATTATTAATATACGACCAGCAGGAATAAGACCTGAACGAATACCATTATTTGT ATGATCAGTAGGTATTACAGCTCTTCTATTATTGTTGTCTTTACCTGTTTTAGCGGGGGCCATTACAATA CTTTTAACTGACCGAAACTTTAATACATCTTTCTTTGATCCTACCGGGGGAGGAGATCCTATTCTATATC AACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Boisea rubrolineata was found to be negative for Wolbachia.
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