CMLM 02

September 10, 2026

Sample information
Picture
Entry by: Lellian Mansour
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda

Methods
Extraction kit net
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard election
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 1: DNA standard

Lane 2:water control

Lane 3:DNA cotrol

Lane 4:wolbaciha (-)

Lane 5: wolbaciha (+)

Lane 6: gross hopper

Lane 7:catapiller

Lane 8:

Lane 9:DNA standard

Lane 10:water control

Lane 11: DNA control

Lane 12:Wolbachia (-)

Lane 13:Wolbachia(+)

Lane 14: Halyini

Lane 15: Northe Metalmark

 
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
> LV-1C2-CO1F TTTNGGTGTGTGAGCAGGAATAATTGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCTGGATCACTAA TTGGAGATGATCAAATTTATAACACTATTGTTACAGCTCATGCCTTTATTATAATTTTTTTTATAGTTATGCCAATTATA ATTGGAGGATTTGGTAATTGATTAATTCCTTTGATATTAGGAGCCCCTGATATAGCTTTCCCACGTATAAATAATATAAG ATTTTGATTACTACCCCCATCATTAACTTTATTAATTTCTAGAAGTATTGTTGAAAACGGAGCAGGAACAGGATGAACAG TTTACCCCCCTCTTTCCTCTAATATTGCCCATAGAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCACCTTGCAGGA ATTTCCTCTATCCTAGGAGCAATTAATTTTATTACAACAATTATCAATATACGAATTAATAATATGTCATTTGATCAAAT ACCTTTATTTGTGTGAGCAGTAGGAATTACTGCTTTATTATTATTATTATCATTACCAGTTTTAGCTGGTGCAATTACTA TATTATTAACTGATCGGAATTTAAATACCTCTTTTTTTGACCCTGCTGGGGGAGGAGACCCAATTCTTTATCAACATTTA TTTTGATTTTNNNNNNNC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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