Lithobius Forficatus (Centipede)

September 10, 2026

Sample information
Picture
Entry by: Sofia N
Location No map location data available.
Collection date 04/08/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found in the grass

Small, many legs, brown
Putative identification Arthropoda Chilopoda Lithobiomorpha Lithobiidae Lithobius Lithobius forficatus

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard election
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Bottom row of gel 1-8 (DNA standard, SNMV1+Wol, SNMV2+Wol, Positive, Negative, DNA control, water control, Empty)

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No band
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GNAGCCTGAGCATCCATAGTAGGGACTGCTCTTAGATTATTAATCCGTCTAGAACTAAGCCAACNNGGAAGTTTAATTGG GGATGATCAAATCTATAATGTCATTGTCACAGCGCATGCTTTTGTTATAATCTTCTTTATAGTGATACCGATCATAATTG GGGGATTTGGTAATTGATTAGTGCCTCTTATATTAGGAGCCCCTGATATAGCCTTTCCTCGACTAAATAACATAAGTTTT TGACTTCTACCCCCCTCCTTCATGCTACTTTTAGGCTCAGCAGCTGTTGAAAATGGTGCTGGAACTGGGTGGACTGTCTA CCCCCCCTTAGCAGCAGGAATATCCCATAGAGGTGGCTCTGTTGACATAACAATTTTCTCTCTCCATCTAGCTGGTATCT CCTCAATCTTAGGGGCTATCAATTTTATCACAACAATTATTAATATACGAACAAGAGGTATAATTTTCGAACGTATACCA CTTTTTGTCTGAGCTGTAAAAATCACAGCTATTTTATTACTTCTTTCCTTACCAGTGTTAGCCGGGGCAATTACTATACT TCTAACTGATCGAAATTTTAACACAAGCTTTTTTGACCCAGCAGGAGGAGGAGACCCTATTTTGTACCAACACCTATTCT GATTTTTTGGTCACCTNNNAAGTTTAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lithobius forficatus was found to be negative for Wolbachia.
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