Wolf Spider (Lycosoidea)

September 10, 2026

Sample information
Picture
Entry by: Gabriel O
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found on sidewalk.

Brown, small, 8 legs, bulbous body, slightly translucent.
Putative identification Arthropoda Arachnida Araneae Lycosidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard election
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

1 DNA standard

2 02 GOHN+SKB1+ 02G0HN+SRB1 – Arth. 1

3 02 GOHN + SKB 2 + 02GOHN+ SRB2 -Arth. 2

4 02 GOHN+SKB3 + 02GOHN+SRB3 -(+) cont.

5 02 GOHN + SKB4 + 02GOHN + SKB4 – (-) cont.

6 02 GOHN+SK + 02G0HN+SK -DNA cont.

7 02 00HN+SK + 02GOHN+SK -water

8 Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Nothing was amplified in our electrophoresis gel so no bands were produced
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lycosidae was found to be negative for Wolbachia.
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