Stagmomantini

Sample information

Picture
Entry by: Haidar N
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found on green leaf of tree looked like small twig, it was brown and stick like.

Putative identification Arthropoda

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard election
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

1 DNA STANDARD

2 O2 GOHN+SKB1+O2GOHN+SRB1-Arth.1

3 O2 GOHN+SKB2+O2GOHN+SRB2-Arth 2

4 O2 GOHN+SKB3+O2GOHN+SRB3-+ cont

5 O2 GOHN +SKB4+O2GOHN+SRB4 — cont

6 O2 GOHN +SK+O2GOHN+SK-DNA cont

7 O2 GOHN+SK+O2GOHN+SK-water

8 empty

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Nothing amplified in gel so no bands produced.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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