Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 09/10/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Found on green leaf of tree looked like small twig, it was brown and stick like. |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard election |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | 1 DNA STANDARD 2 O2 GOHN+SKB1+O2GOHN+SRB1-Arth.1 3 O2 GOHN+SKB2+O2GOHN+SRB2-Arth 2 4 O2 GOHN+SKB3+O2GOHN+SRB3-+ cont 5 O2 GOHN +SKB4+O2GOHN+SRB4 — cont 6 O2 GOHN +SK+O2GOHN+SK-DNA cont 7 O2 GOHN+SK+O2GOHN+SK-water 8 empty |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Nothing amplified in gel so no bands produced. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |


ABRHS 2026 – Ant – 12-3 Synemosyna Formica
ZETNJM Hymenoptera
CON-SCA Hemiptera
CONDJ Hemiptera