North American Spur Throated Grasshopper ( Genus Melanoplus )

September 10, 2026

Sample information
Picture
Entry by: Jayanne R
Location
Approximate location: 36.14, -86.80
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Red legs, green body,  it was on a bush with another grasshopper. The bush had stick like foliage.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard election
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

1: DNA standard

2: Arthropod #1 WOL + COL 1

3: Arthropod #2 WOL + COL 1

4: WOL POS Wol + COL 1

5: WOL Neg Wol + COL 1

6: DNA control WOl +COL 1

7: Water control Wol +COL 1

8: empty

9: DNA standard

10: Arthropod #1 WOL + COL 1

11: Arthropod #2 WOL + COL 1

12: WOL POS Wol + COL 1

13: WOL Neg Wol + COL 1

14: DNA control WOl +COL 1

15: Water control Wol +COL 1

16: empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We didn’t get any bands on our gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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