Tinea (Moth)

September 10, 2026

Sample information
Picture
Entry by: Autumn B
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit Qiagen Blood and Tissue kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system agarose gel electrophoresis
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
Group 2D (Aqsa and Jeanne)
Lanes 1-8

Lane 1- DNA Standard

Lane 2- Arthropod #1 Wol + COI1

Lane 3- Arthropod #2 Wol + COI1

Lane 4- Wol Pos Wol + COI1

Lane 5- Wol Neg Wol + COI1

Lane 6- gDNA control Wol + COI1

Lane 7- water control Wol + COI1

Lane 8- Empty
Group 2E ( Autumn and Katelynn)
Lane 9- DNA Standard

Lane 10- Arthropod #1 Wol + COI1

Lane 11- Arthropod #2 Wol + COI1

Lane 12- Wol Pos Wol + COI1

Lane 13- Wol Neg Wol + COI1

Lane 14- gDNA control Wol + COI1

Lane 16- water control Wol + COI1

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

A band was produced with the PCR sample when Gel Electrophoresis was done.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
GGATCTGAGCCGGAATAATTGGAACCTCTTTAAGATTAATAATTCGAGCTGAATTAGGAGCTCCAGGACATTTAATTGGA GACGATCAAATTTATAATACTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTAATGCCAATTATAATTGG AGGATTTGGAAATTGATTAGTGCCTTTAATATTAGGGGCTCCAGATATAGCTTTCCCCCGAATAAACAACATAAGATTTT GAATATTACCCCCTTCCATTATATTACTCATCTCAAGAAGAGTGGTTGAAAATGGAGCTGGTACAGGATGAACAGTATAT CCCCCACTTTCATCAAATATTTCTCATAGAGGAAGATCTGTAGACTTAGCTATTTTTTCATTACATTTAGCTGGAATTTC ATCTATTTTAGGAGCAATTAATTTTATCACAACAATTATTAATATACGATTAAATAACATATCATTTGACCAATTACCAT TATTTGTTTGAGCAGTAGGTATTACAGCATTCTTACTATTATTATCATTACCTGTATTAGCAGGTGCAATTACTATATTA TTAACAGATCGAAATTTAAATACATCCTTTTTTGACCCTGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTG ATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be postive for Wolbachia.
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