Red winged beetle

September 10, 2026

Sample information
Picture
Entry by: Avery L
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

found in the net at the bottom, not moving much. Only moved when we touched it.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR-SAFE
Gel images
Protocol notes

lane 9 – DNA St

lane 10 – Arthropod #1 (Wol + COL1) (BSAL1)

lane 11 – Arthropod #2 (Wol + COL1) (BSAL2)

lane 12 – Wol (+) (Wol + COL1)

lane 13 – Wol (-) (Wol + COL1)

lane 14 – DNA control (Wol + COL1)

lane 15 – Water control (Wol + COL1)

lane 16 – Empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

It is missing a double line
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
TTTTNGNNNNTNATCTGGTATAGTAGGTTCATCTTTAAGATGAATTATTCGTGTAGAATTAGGGCAACCTGGTAGATTTA TTGGGGATGATCAAACATATAATGTAATTGTAACAGCACATGCTTTTATTATAATTTTCTTTATAGTTATGCCAATTATG ATTGGTGGATTTGGGAATTGATTAGTGCCTTTAATAATTGGGGCCCCAGATATAGCATTTCCTCGAATAAATAATATAAG ATTTTGACTTTTACCTCCTTCCCTTACTTTATTACTTGCAAGTAGTATAGTTGAAAGAGGAGCGGGAACTGGATGAACAG TTTATCCCCCTCTATCAAGAAATTTATCCCATAGAGGTGCCTCTGTAGATTTAGCAATTTTTTCATTACATTTAGCAGGT GTATCATCAATTTTAGGGGCTATTAATTTTATTTCAACTATTATTAATATACGGCCAGCAGGAATAAGACCTGAACGAAT ACCATTATTTGTATGATCAGTAGGTATTACAGCTCTTCTATTATTGTTGTCTTTACCTGTTTTAGCGGGGGCCATTACAA TACTTTTAACTGACCGAAACTTTAATACATCTTTCTTTGATCCTACCGGGGGAGGAGATCCTATTCTATATCAACATTTA TTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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