Stink Bug ( Order Hemiptera)

September 10, 2026

Sample information
Picture
Entry by: Damian M
Location
Approximate location: 40.56, -75.40
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Found in Bush, not easily visible caught with sweep net
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Lane 1: DNA Standard

Lane 2: Arthropod #1 Wol + Col1

Lane 3: Arthropod #2 Wol +Col1

Lane 4: Wol pos Wol + Col1

Lane 5: Wol neg Wol + Col1

Lane 6: gDNA control + Col1

Lane 7: Water Control Wol + Col1

Lane 8: EMPTY

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
ATNAGCAGGAATAGTGGGGTCCGCTATGAGAATTATTATTCGTATTGAATTAGGACAACCAGGAAGATTTATTGGAGATG ATCAAATCTATAATGTTGTAGTTACTGCTCACGCATTCGTTATAATTTTTTTTATAGTAATACCAATTATAATTGGAGGA TTTGGTAACTGACTAGTGCCATTAATAATTGGAGCTCCTGATATAGCATTCCCTCGAATAAATAATATAAGATTCTGGTT ATTACCCCCTTCAATTACTTTATTAATAATAAGTAGATTAGCTGAATCAGGGGCTGGAACCGGCTGAACTGTATATCCCC CACTATCAAGTAATCTATCCCACAGAGGAGCATCAGTAGACTTGGCTATCTTTTCCTTACACTTAGCAGGTGTTTCTTCA ATTCTAGGAGCTGTAAATTTCATTTCAACAATTATAAATATACGACCCGAAGGAATAATCCCAGAACGAATCCCCTTATT CGTATGATCAGTAGGAATTACAGCACTATTATTACTACTTTCCCTTCCCGTATTAGCAGGAGCTATTACTATATTATTAA CCGATCGCAACTTTAATACATCCTTCTTTGATCCTTCAGGAGGGGGGGATCCTATCTTGTATCAGCATCTATTCTGATTT T
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be negative for Wolbachia.
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