Grasshopper

September 10, 2026

Sample information
Picture
Entry by: Bassam Bittar
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Its has a lot little hair legs when u look under a scope
Putative identification

Methods
Extraction kit Qiagen kit for blood and tissues.
DNA extraction location Lab
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

TTNGNATATGATCTGGTATAGTAGGTTCATCTTTAAGATGAATTATTCGTGTAGAATTAGGGCAACCTGGTAGATTTATT
GGGGATGATCAAACATATAATGTAATTGTAACAGCACATGCTTTTATTATAATTTTCTTTATAGTTATGCCAATTATGAT
TGGTGGATTTGGGAATTGATTAGTGCCTTTAATAATTGGGGCCCCAGATATAGCATTTCCTCGAATAAATAATATAAGAT
TTTGACTTTTACCTCCTTCCCTTACTTTATTACTTGCAAGTAGTATAGTTGAAAGAGGGGCGGGAACTGGATGAACAGTT
TATCCCCCTCTATCAAGAAATTTATCCCATAGAGGTGCCTCTGTAGATTTAGCAATTTTTTCATTACATTTAGCAGGTGT

lane 1 DNA standard

Lane 2 water control

Lane 3 DNA control

Lane 4 Wol neg

Lane 5 wol pos

Lane 6 grass hopper

Lane 7 Caterpillar

Lane 8 empty

Lane 9 DNA standard

Lane 10 water control

Lane 11 DNA control

Lane 12 wol neg

Lane 13 wol pos

Lane 14 Halgiani

Lane 15 anathern metalmark

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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