JWO 01 – Plain Tailed Hanging Thief

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Sitting on flower in HACC Pollinator Garden

Orange, black vert stripes, long legs, piercing mouth parts, 1 inch long.
Putative identification Arthropoda Insecta Diptera Asilidae Diogmites Diogmites neoternatus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex pcr and minione gel procedures. Refer to label 1 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Band in arthropod CO1, no band for Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diogmites neoternatus was found to be negative for Wolbachia.
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