JWO 02 – Differential Grasshopper

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Sitting on stem in HACC Pollinator Garden

Mostly green, outside of legs had black arrows.
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus Melanoplus differentialis

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex pcr and minione gel procedures. Refer to label 2 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Band seen in arthropod CO1 but not Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Melanoplus differentialis was found to be negative for Wolbachia.
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