JWO 03 – Chickweed Geometer

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On flower in HACC Pollinator Garden

Orange moth, red lines, 2 red dots on wings
Putative identification Arthropoda Insecta Lepidoptera Geometridae Haematopis Haematopis grataria

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex pcr and minione gel procedures. Refer to label 3 on gel image.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Bands seen on both CO1 and Wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Haematopis grataria was found to be postive for Wolbachia.
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