JWO 12 – Narrow-winged Damselfly (Female)

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

4 wings, 3 black vert stripes on thorax, grey w/ orange hues,

Flying around in HACC Pollinator Garden
Putative identification Arthropoda Insecta Odonata Coenagrionidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 12 on gel image.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

2 clear defined bands, 1 in arthropod CO1 gene and 1 at Wolbachia 16S rRNA gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coenagrionidae was found to be postive for Wolbachia.
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