JWO 04 – Grasshopper Nymph

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On grass near HACC Pollinator Garden

small green grasshopper, large hind femurs, chewing mouth parts
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 4 on gel image.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Faint band in the arthropod CO1. 2 gels ran with sample, same results occured.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Melanoplus was found to be negative for Wolbachia.
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